Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine Sulfate) in the process of vaccine production
Identifieur interne : 000B10 ( Main/Exploration ); précédent : 000B09; suivant : 000B11Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine Sulfate) in the process of vaccine production
Auteurs : Yoshihiro Sakoda ; Masatoshi Okamatsu ; Norikazu Isoda ; Naoki Yamamoto ; Koichi Ozaki ; Yasuto Umeda ; Shigeyuki Aoyama ; Hiroshi Kida [Japon]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2012-07.
English descriptors
- Teeft :
- Allantoic, Avian, Avian viruses, Blackwell publishing asia, Chicken eggs, Commercial vaccine, Cynomolgus macaques, Dextran sulfate, Disease control, Eluate, Elution, Elution buffer, Elution fractions, Elution method, Embryonated chicken eggs, Fields virology, Further investigation, Future pandemic situations, Gradient elution, Graduate school, Hokkaido university, Human viruses, Lippincott williams wilkins, Matrix protein, Nacl, Nacl concentration, Nacl gradient, Optical density, Pandemic virus, Phosphate buffer, Practical method, Present study, Protective immunity, Protein marker, Protein recoveries, Recovery rate, Recovery rates, Sapporo japan, Separate fraction tubes, Sodium dodecyl sulfate polyacrylamide, Sulfate, Sulfate bead, Sulfate beads, Sulfate columns, Total amount, Total protein, Vaccine, Vaccine production, Vaccine strains, Veterinary medicine, Viral proteins, Virus, Virus particles, Virus recovery rate, Virus strain, Virus strains.
Abstract
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine Sulfate) was assessed for purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful for removing chicken egg‐derived impurities from allantoic fluids containing influenza viruses; the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that use of Cellufine Sulfate is a practical method for primary purification of influenza viruses in the process of influenza vaccine production.
Url:
DOI: 10.1111/j.1348-0421.2012.00468.x
Affiliations:
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unit="issue">7</biblScope><biblScope unit="page" from="490">490</biblScope><biblScope unit="page" to="495">495</biblScope><biblScope unit="page-count">6</biblScope><publisher>Blackwell Publishing Asia</publisher><pubPlace>Melbourne, Australia</pubPlace><date type="published" when="2012-07">2012-07</date></imprint><idno type="ISSN">0385-5600</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0385-5600</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Allantoic</term><term>Avian</term><term>Avian viruses</term><term>Blackwell publishing asia</term><term>Chicken eggs</term><term>Commercial vaccine</term><term>Cynomolgus macaques</term><term>Dextran sulfate</term><term>Disease control</term><term>Eluate</term><term>Elution</term><term>Elution buffer</term><term>Elution fractions</term><term>Elution method</term><term>Embryonated chicken eggs</term><term>Fields virology</term><term>Further investigation</term><term>Future 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particles</term><term>Virus recovery rate</term><term>Virus strain</term><term>Virus strains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Affinity chromatography using sulfated, spherical cellulose beads (Cellufine Sulfate) was assessed for purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful for removing chicken egg‐derived impurities from allantoic fluids containing influenza viruses; the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that use of Cellufine Sulfate is a practical method for primary purification of influenza viruses in the process of influenza vaccine production.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Aoyama, Shigeyuki" sort="Aoyama, Shigeyuki" uniqKey="Aoyama S" first="Shigeyuki" last="Aoyama">Shigeyuki Aoyama</name><name sortKey="Isoda, Norikazu" sort="Isoda, Norikazu" uniqKey="Isoda N" first="Norikazu" last="Isoda">Norikazu Isoda</name><name sortKey="Okamatsu, Masatoshi" sort="Okamatsu, Masatoshi" uniqKey="Okamatsu M" first="Masatoshi" last="Okamatsu">Masatoshi Okamatsu</name><name sortKey="Ozaki, Koichi" sort="Ozaki, Koichi" uniqKey="Ozaki K" first="Koichi" last="Ozaki">Koichi Ozaki</name><name sortKey="Sakoda, Yoshihiro" sort="Sakoda, Yoshihiro" uniqKey="Sakoda Y" first="Yoshihiro" last="Sakoda">Yoshihiro Sakoda</name><name sortKey="Umeda, Yasuto" sort="Umeda, Yasuto" uniqKey="Umeda Y" first="Yasuto" last="Umeda">Yasuto Umeda</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></noCountry><country name="Japon"><noRegion><name sortKey="Kida, Hiroshi" sort="Kida, Hiroshi" uniqKey="Kida H" first="Hiroshi" last="Kida">Hiroshi Kida</name></noRegion><name sortKey="Kida, Hiroshi" sort="Kida, Hiroshi" uniqKey="Kida H" first="Hiroshi" last="Kida">Hiroshi Kida</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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